A Note on Abnormalities induced in Mouse Embryos by Trypan Blue

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Trypan blue induced teratogenesis of rat embryos cultivated in vitro.

Trypan blue is known to produce embryonic abnormalities in a wide variety of animals, including rats (Gillman, Gilbert, Gillman & Spence, 1948), mice (Waddington & Carter, 1953; Hamburgh, 1954), amphibians (Waddington & Perry, 1956), chickens (Beaudoin & Wilson, 1958;Stephan & Sutter, 1961), rabbits (Ferm, 1956), and hamsters (Ferm, 1958). Studies on the teratogenic action of this dye have also...

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The Effects of Trypan Blue on Chick Embryos

VARIOUS malformations in rats and mice have been observed to be caused by administration of the vital dye, Trypan blue (Gillman, Gilbert, Gillman, & Spence, 1948; Fox & Goss, 1956, 1957; Hamburgh, 1952, 1954; Waddington & Carter, 1952, 1953). Recently Waddington & Perry (1956) reported a teratogenic effect of Trypan blue on Amphibian embryos. The present paper deals with the effects of this dye...

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Teratogenic effects of trypan blue on hamster embryos.

L I T T L E information is available in the literature on the susceptibility of the hamster embryo to teratogens. Desoxycorticosterone acetate apparently has some teratogenic activity (Tedford & Risely, 1950), but the degree and type of abnormality produced by this agent in the hamster have not been recorded. Orsini (1952) has described naturally-occurring urogenital anomalies which appear in f...

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Anemia with positive direct Coombs' test induced by trypan blue.

as evidence of autoimmune phenomena. The experimental production of positive antiglobulin tests by drugs and chemical agents, however, has suggested that nonimmunologic mechanisms may also be responsible for protein coating of erythrocytes.2’3 During attempts to produce tumors of the reticuloendothelial system in rats by injection of trypan blue, an anemia, chacacterized by increased ervthrocyt...

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Trypan blue exclusion assay by flow cytometry

Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the...

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ژورنال

عنوان ژورنال: Development

سال: 1953

ISSN: 1477-9129,0950-1991

DOI: 10.1242/dev.1.2.167